Difference between revisions of "Part:BBa K1334002"
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===Usage and Biology===
 
===Usage and Biology===
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A gfp reporter gene was added downstream so that we can know whether the P-formaldehyde works or not in the presence of formaldehyde by detecting the relative fluorescence intensity.
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[[File:P-Formaldehyde+GFP.png]]
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Figure 3 A GFP reporter is added downstream of the formaldehyde promoter.
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We incubated E.coli strain DH5a with the above plasmid （P-formaldehyde plus GFP）and stimulated the experimental group with 1mM formaldehyde when the OD600 value reached approximately one, which means E.coli comes into mid-log phase. And then we collected bacteria at certain time to measure the fluorescence and the absorbance.
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From the Figure 4, we can see significant increase in relative fluorescence intensity after formaldehyde stimulation, which shows that our coloration system works well. The Growth Condition curve in Figure 5 shows that the difference of the fluorescence/absorbance is not generated by the absorbance difference.
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[[File:Figure 4  The function test of the formaldehyde promoter which shows our part BBa_K1334002 works well.png]]
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[[File:Figure 5  The growth condition of E.coli strain DH5a after formaldehyde stimulation.png]]
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Revision as of 04:03, 17 October 2014

HxlR+P-formaldehyde

A right promoter which can sense formaldehyde.There is a left promoter which can erpress the HxlR protein.The HxlR protein can enhance the function of the P-formaldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]