Difference between revisions of "Part:BBa K1444023"
 
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===Usage and Biology===
 
===Usage and Biology===
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This part is one of a series of designed repressors used by the 2014 Calgary iGEM team (<partinfo>BBa_K1444002</partinfo>), cloned in front of the C2-P22 repressor protein <partinfo>BBa_C0053</partinfo>. Though this construct is itself repressible, our team only used it as a constitutive generator. We were able to show that this construct prevented expression of our reported construct <partinfo>BBa_K1444022</partinfo>. See that page for more details.
+
This part is one of a series of designed repressors used by the 2014 Calgary iGEM team (<partinfo>BBa_K1444002</partinfo>), cloned in front of the C2-P22 repressor protein <partinfo>BBa_C0053</partinfo>. Though this construct is itself repressible, our team only used it as a constitutive generator. We were able to show that this construct prevented expression of our reporter construct <partinfo>BBa_K1444022</partinfo>. See that page for more details.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 03:33, 17 October 2014

Constitutive repressor generator

Constitutive expression of the repressor protein C2-P22.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 485

Usage and Biology

This part is one of a series of designed repressors used by the 2014 Calgary iGEM team (BBa_K1444002), cloned in front of the C2-P22 repressor protein BBa_C0053. Though this construct is itself repressible, our team only used it as a constitutive generator. We were able to show that this construct prevented expression of our reporter construct BBa_K1444022. See that page for more details.