Difference between revisions of "Part:BBa K1362021:Design"

(Source)
(Design Notes)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
???
+
The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.
 
+
  
 +
For more information on the circularization see the [[Part:BBa_K1362000|Intein Circularization Construct]]
  
 
===Source===
 
===Source===

Latest revision as of 02:52, 17 October 2014


RBS + Circular Xylanase with His6 linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557
    Illegal SapI.rc site found at 611


Design Notes

The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.

For more information on the circularization see the Intein Circularization Construct

Source

See the Basic Xylanase Part

References