Difference between revisions of "Part:BBa K1362021:Design"

 
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===Design Notes===
 
===Design Notes===
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The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.
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For more information on the circularization see the [[Part:BBa_K1362000|Intein Circularization Construct]]
  
 
===Source===
 
===Source===
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See the [[Part:BBa_K1362441:Design#Source|Basic Xylanase Part]]
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===References===
 
===References===

Latest revision as of 02:52, 17 October 2014


RBS + Circular Xylanase with His6 linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557
    Illegal SapI.rc site found at 611


Design Notes

The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.

For more information on the circularization see the Intein Circularization Construct

Source

See the Basic Xylanase Part

References