Difference between revisions of "Part:BBa K1321163"
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This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-NiBP-part-table.PNG]] | This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-NiBP-part-table.PNG]] | ||
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Latest revision as of 01:54, 17 October 2014
NiBP driven by T7 promoter
**Important note**: Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part. We hope that this part will still be useful, since the error could be corrected relatively easily by a team who wishes to it by site-directed-mutagenesis PCR to insert the missing nucleotide.
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below: 
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000COMPATIBLE WITH RFC[1000]