Difference between revisions of "Part:BBa K1321009"
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<partinfo>BBa_K1321009 short</partinfo> | <partinfo>BBa_K1321009 short</partinfo> | ||
| − | + | '''**Important note**''': Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from ''Heliobacter pylori'' (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part. To correct the frame-shift, a 'C' needs to be added between bases 49 and 50. | |
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| + | This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information). | ||
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| + | For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162). We used this part to create a [http://2014.igem.org/Team:Imperial/Functionalisation library of fusion proteins with cellulose-binding domains]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K1321009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1321009 SequenceAndFeatures</partinfo> | ||
Latest revision as of 01:37, 17 October 2014
Nickel Binding Protein (NiBP) Freiburg format (RFC[25])
**Important note**: Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part. To correct the frame-shift, a 'C' needs to be added between bases 49 and 50.
This part is BBa_K1151001 in Frieburg (RFC[25]) format to allow for easy use in fusion proteins. Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown. It is a histidine-rich metal binding protein which binds up to 5 Ni2+ ions (please see the original BBa_K1151001 biobrick page from team Università del Salento Lecce 2013 for more detailed information).
For expression purposes this part is available with T7 promoter (BBa_K1321163) or LacI promoter (BBa_K1321162). We used this part to create a [http://2014.igem.org/Team:Imperial/Functionalisation library of fusion proteins with cellulose-binding domains].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]