Difference between revisions of "Part:BBa K1323014"

Revision as of 01:11, 17 October 2014

Xylose Inducible Promoter for Staphylococcus aureus

This sequence contains the T5X xylose inducible promoter and the xylose promoter repressor, XylR, for Staphylococcus. It has been shown by Forsyth et al that 2% xylose in LB is sufficient to generate significant expression downstream of this promoter (Forsyth et al, 2002). In the absence of xylose, XylR represses activity by binding to the promoter region. Furthermore, glucose was shown to repress the activity of this promoter (Wieland et al, 1995). Our team obtained the sequence for this part from Dr. Donegan from the Ambrose Lab at The Geisel School of Medicine, Dartmouth. We were able to get this part synthesized from BioBasic. By doing so, we were able to remove 3 illegal sites.

Forsyth RA, et al., 2002. A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol 43:1387–1400

Wieland, K.P, Wieland, B. Gotz, F. 1995. A promoter screening plasmid and xylose inducible glucose-repressible expression vectors for Staphylococcus carnosus. Gene. 158: 91- 96.

Sequence and Features