Difference between revisions of "Part:BBa K1420006"

Revision as of 00:40, 17 October 2014

phsABC Generator

Summary

The (phsABC) gene from Salmonella enterica serovar Typhimurium LT2 encodes thiosulfate reductase, which catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate for heavy metal removal by precipitation.

Experimental Results

In order to measure the thiosulfate reducing activity of phsABC of NaS₂O₃ to H₂S, the gene was first inserted into the pBBRBB vector with a constitutive P_lac promoter and transformed into K12 strain E. coli cells. As a control, pBBRBB::GFP was tested under the same conditions. The pBBRBB:phsABC K12 and pBBRBB::GFP K12 cells were grown in three test tubes each containing heavy metal tryptone medium as well as 3mM NaS₂O₃. A third set of test tubes were set up with the same contents except without cells as an additional negative control. After 24 hours, the tubes appeared as displayed in Figure 3. The K12 cells contained The exact amount of H₂S present in each of three different sets of tubes was then measured using a hydrogen sulfide assay (Figure 4). The numerical results are displayed in Figure 5

Figure 3.

Figure 4. 1:10 dilution of cell samples in Cline's Reagent used for hydrogen sulfide assays.

References

Sequence and Features