Difference between revisions of "Part:BBa K1442040"
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Revision as of 22:25, 16 October 2014
MS2 bacteriophage coat protein
The MS2 bacteriophage coat protein is a translational repressor [4] that binds the MS2 hairpin to encapsidate the genome and represses replicase synthase translation [1]. Exploiting the MS2 box-protein binding relationship is known as MS2 tagging.
Isaacs et al [2] noted that the MS2 protein binding box is of use for engineering RNA transcriptional activators via the DNA-protein binding interactions and the hairpin has thus been shown that the MS2 box can be used to drive expression of proteins when localised to a promoter (e.g. the MS2 protein is fused to a DNA-binding protein and the MS2 box is localised to its target promoter, driving expression).
Furthermore, Auslander et al [3] also showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of a informational processing system without researcher input.
Usage and Biology
The MS2 coat-protein was included in our replicon.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 255
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 255
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 255
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 255
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 255
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 292