Difference between revisions of "Part:BBa K1442040"

(Usage and Biology)
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<p>The MS2 coat-protein was included in our replicon.</p>  
 
<p>The MS2 coat-protein was included in our replicon.</p>  
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Revision as of 22:25, 16 October 2014

MS2 bacteriophage coat protein

The MS2 bacteriophage coat protein is a translational repressor [4] that binds the MS2 hairpin to encapsidate the genome and represses replicase synthase translation [1]. Exploiting the MS2 box-protein binding relationship is known as MS2 tagging.

Isaacs et al [2] noted that the MS2 protein binding box is of use for engineering RNA transcriptional activators via the DNA-protein binding interactions and the hairpin has thus been shown that the MS2 box can be used to drive expression of proteins when localised to a promoter (e.g. the MS2 protein is fused to a DNA-binding protein and the MS2 box is localised to its target promoter, driving expression).

Furthermore, Auslander et al [3] also showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of a informational processing system without researcher input.

Usage and Biology

The MS2 coat-protein was included in our replicon.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 255
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 292