Difference between revisions of "Part:BBa K1463520"

 
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<partinfo>BBa_K1463520 short</partinfo>
 
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Part E1010, RFP, in reverse.
 
Part E1010, RFP, in reverse.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] with transcription driven by the reverse promoter [https://parts.igem.org/Part:BBa_K1463501 BBa_K1463501] in [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000].
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See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details.
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[[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing  [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [https://parts.igem.org/Part:BBa_I742130 BBa_I742130] reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000]. The image was scanned using a GE Healthcare Typhoon FLA9500 using the 532 nm laser and LPG filter.]]
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K1463520 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1463520 SequenceAndFeatures</partinfo>
  

Latest revision as of 22:08, 16 October 2014

E1010 RFP in reverse

Part E1010, RFP, in reverse.

Usage and Biology

This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site BBa_K1463560 with transcription driven by the reverse promoter BBa_K1463501 in BBa_K1463000.

See BBa_K1463560 and BBa_K1463000 for more details.

Fig. 1: Red fluorescence of colonies containing BBa_K1463560 or BBa_I742130 reversed RBSs "downstream" of the reversed rfp gene BBa_K1463520 transcribed from the PhiC31 integrase switch BBa_K1463000. The image was scanned using a GE Healthcare Typhoon FLA9500 using the 532 nm laser and LPG filter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]