Difference between revisions of "Part:BBa I742130:Experience"

 
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===Applications of BBa_I742130===
 
===Applications of BBa_I742130===
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===Glasgow 2014 experience===
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We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520]. This can be seen in Fig. 1 below.
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It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560].
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[[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing  [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [https://parts.igem.org/Part:BBa_I742130 BBa_I742130] reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000]. The image was scanned using a GE Healthcare Typhoon FLA9500 using the 532 nm laser and LPG filter.]]
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Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
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BBa_I742130  calculated translation initiation rate 495.33
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BBa_K1463560  calculated translation initiation rate 12,204.43
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 22:03, 16 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742130

Glasgow 2014 experience

We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick BBa_K1463520. This can be seen in Fig. 1 below.

It gave a lower fluorescence than our designed reverse RBS BBa_K1463560.


Fig. 1: Red fluorescence of colonies containing BBa_K1463560 or BBa_I742130 reversed RBSs "downstream" of the reversed rfp gene BBa_K1463520 transcribed from the PhiC31 integrase switch BBa_K1463000. The image was scanned using a GE Healthcare Typhoon FLA9500 using the 532 nm laser and LPG filter.

Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.

BBa_I742130 calculated translation initiation rate 495.33

BBa_K1463560 calculated translation initiation rate 12,204.43

User Reviews

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