Difference between revisions of "Part:BBa K1316018:Design"

 
 
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<partinfo>BBa_K1316018 short</partinfo>
 
<partinfo>BBa_K1316018 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The sequence was screened for illegal iGEM restriction sites
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The part contains an RBS upstream the gene in order to ensure proper protein translation.
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The part was PCRed in a way that it contains the beginning of a constitutive promoter after the gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.
  
  
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===Source===
 
===Source===
  
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It comes from the iGEM BioBrick BBa_K914003
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The CDS of csgB, as well as the RBS, contained in this part were PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011.
  
 
===References===
 
===References===

Latest revision as of 22:03, 16 October 2014

RBS + csgB curli-forming gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 516
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains an RBS upstream the gene in order to ensure proper protein translation. The part was PCRed in a way that it contains the beginning of a constitutive promoter after the gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.


Source

The CDS of csgB, as well as the RBS, contained in this part were PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011.

References