Difference between revisions of "Part:BBa K1554000:Design"

(Design Notes)
(Design Notes)
 
Line 9: Line 9:
 
This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.
 
This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.
  
It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (AATG and GCTT). This part was sent to the Registry of Parts using [https://parts.igem.org/Part:BBa_K1467100 RFP coding device - Golden Gate Module Flipper].
+
It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (GGAG and AATG). This part was sent to the Registry of Parts using [https://parts.igem.org/Part:BBa_K1467100 RFP coding device - Golden Gate Module Flipper].
  
 
===Source===
 
===Source===

Latest revision as of 21:35, 16 October 2014


TA29 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.

It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (GGAG and AATG). This part was sent to the Registry of Parts using RFP coding device - Golden Gate Module Flipper.

Source

Genomic DNA from Nicotiana tabacco.

References

[1] Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., and Goldberg, R.B. (1990). lnduction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 347, 737–741.

[2] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333

[3] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278

[4] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130

[5] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.