Difference between revisions of "Part:BBa K1554000:Design"

(→‎References)
(→‎Design Notes)
 
(8 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
BioBrick compatible
+
This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.
−
 
+
  
 +
It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (GGAG and AATG). This part was sent to the Registry of Parts using [https://parts.igem.org/Part:BBa_K1467100 RFP coding device - Golden Gate Module Flipper].
  
 
===Source===
 
===Source===
−
 
+
Genomic DNA from ''Nicotiana tabacco''.
−
Amplified from genomic DNA of Nicotiana tabacco.
+
  
 
===References===
 
===References===
−
[1] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333
 
  
−
[2] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278
+
[1] Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., and Goldberg, R.B. (1990). lnduction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 347, 737–741.
 +
 
 +
[2] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333
 +
 
 +
[3] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278
  
−
[3] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130
+
[4] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130
  
−
[4] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.
+
[5] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.

Latest revision as of 21:35, 16 October 2014


TA29 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.

It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (GGAG and AATG). This part was sent to the Registry of Parts using RFP coding device - Golden Gate Module Flipper.

Source

Genomic DNA from Nicotiana tabacco.

References

[1] Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., and Goldberg, R.B. (1990). lnduction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 347, 737–741.

[2] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333

[3] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278

[4] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130

[5] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.