Difference between revisions of "Part:BBa K1351037:Design"
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===Design Notes===
 
===Design Notes===
  
−
This part has been artificially synthesized. Mutated illegal restriction sites. These sites have been mutated with regard to the codon usage of Bacillus subtilis.
+
This part has been artificially synthesized with mutated illegal restriction sites. These sites have been mutated with regard to the codon usage of Bacillus subtilis.
  
 +
===Source===
  
 +
This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.
  
−
===Source===
+
P2_fwd: GATCGAATTCGCGGCCGCTTCTAGAG'''GCATTTATTTTCCAATTTTTCTTAACTAG'''
 +
 
 +
P2_rev: GATCACTAGTA'''CCTCACTGTTATTATACGATTTAGTAC'''
  
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artificially synthesized
+
Annealing sequences are written in bold.
  
 
===References===
 
===References===
 +
Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.

Latest revision as of 21:29, 16 October 2014

P2-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been artificially synthesized with mutated illegal restriction sites. These sites have been mutated with regard to the codon usage of Bacillus subtilis.

Source

This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

P2_fwd: GATCGAATTCGCGGCCGCTTCTAGAGGCATTTATTTTCCAATTTTTCTTAACTAG

P2_rev: GATCACTAGTACCTCACTGTTATTATACGATTTAGTAC

Annealing sequences are written in bold.

References

Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.