Difference between revisions of "Part:BBa K1351038:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1351038 short</partinfo> | <partinfo>BBa_K1351038 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | artificially synthesized | + | This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. |
| + | P3_fwd: GATCGAATTCGCGGCCGCTTCTAGAG[[GTACAATCTTTTATCATTATATTGCCTAAC]] | ||
| + | P3_rev: GATCACTAGTA[[CTCTGTGATCTAGTTATATTAAAACATGC | ||
| + | ]] | ||
| + | Annealing sequences are underlined | ||
===References=== | ===References=== | ||
Revision as of 21:24, 16 October 2014
P3-promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Several mutated illegal restriction sites. The mutated codons have been optimized regarding the codon usage of Bacillus subtilis.
Source
This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.
P3_fwd: GATCGAATTCGCGGCCGCTTCTAGAGGTACAATCTTTTATCATTATATTGCCTAAC P3_rev: GATCACTAGTA[[CTCTGTGATCTAGTTATATTAAAACATGC ]] Annealing sequences are underlined