Difference between revisions of "Part:BBa K1470003"

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===Usage and Biology===
 
Retro viral vectors provide an optimal gene delivery system. They are able to transport large DNA fragments and efficiently integrate them stable in the genome. However, high gene expression levels are often absent [1].  
 
Retro viral vectors provide an optimal gene delivery system. They are able to transport large DNA fragments and efficiently integrate them stable in the genome. However, high gene expression levels are often absent [1].  
 
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It is known that the Woodchuck Hepatitis Virus contains a regulatory RNA sequence, which promotes viral gene expression. When fused downstream to a gene, the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)creates a tertiary structure, which enhances the export of unspliced mRNA from the nucleus into the cytoplasm [2].
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It is known that the Woodchuck Hepatitis Virus contains a regulatory RNA sequence, which promotes viral gene expression. When fused downstream to a gene, the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) creates a tertiary structure, which enhances the export of unspliced mRNA from the nucleus into the cytoplasm [2].<br>
 
Klein <i>et al.</i> showed, that WPRE boosts gene expression level of enhanced green fluorescent protein (EGFP) or viral titres up to five times [3].
 
Klein <i>et al.</i> showed, that WPRE boosts gene expression level of enhanced green fluorescent protein (EGFP) or viral titres up to five times [3].
 
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[3] KLEINA, R., RUTTKOWSKIB, B., KNAPP, E., SALMONSA, B., GUENZBURG, W.H., HOHENADL, C.: WPRE-mediated enhancement of gene expression is promoter and cell line specific. Gene. 2006 May 10;372:153-61
 
[3] KLEINA, R., RUTTKOWSKIB, B., KNAPP, E., SALMONSA, B., GUENZBURG, W.H., HOHENADL, C.: WPRE-mediated enhancement of gene expression is promoter and cell line specific. Gene. 2006 May 10;372:153-61
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1470003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1470003 parameters</partinfo>
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Latest revision as of 20:05, 16 October 2014

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)

Usage and Biology

Retro viral vectors provide an optimal gene delivery system. They are able to transport large DNA fragments and efficiently integrate them stable in the genome. However, high gene expression levels are often absent [1].
It is known that the Woodchuck Hepatitis Virus contains a regulatory RNA sequence, which promotes viral gene expression. When fused downstream to a gene, the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) creates a tertiary structure, which enhances the export of unspliced mRNA from the nucleus into the cytoplasm [2].
Klein et al. showed, that WPRE boosts gene expression level of enhanced green fluorescent protein (EGFP) or viral titres up to five times [3].

References

[1] ZUFFEREY, R., DONELLO, J.E., TRONO, D., HOPE, T.J.: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of Transgenes Delivered by Retroviral Vectors. J Virol. Apr 1999; 73(4): 2886–2892.
[2] POPA, I., HARRIS, M.E., DONELLO, J.E., HOPE, T.J.: CRM1-dependent function of a cis-acting RNA export element. Mol Cell Biol. 2002 Apr;22(7):2057-67.
[3] KLEINA, R., RUTTKOWSKIB, B., KNAPP, E., SALMONSA, B., GUENZBURG, W.H., HOHENADL, C.: WPRE-mediated enhancement of gene expression is promoter and cell line specific. Gene. 2006 May 10;372:153-61


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]