Difference between revisions of "Part:BBa K1351019"

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<partinfo>BBa_K1351019 short</partinfo>
 
<partinfo>BBa_K1351019 short</partinfo>
  
Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br>
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Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br>
 
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br>
 
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br>
 
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
 
Suffix: TACTAGTAGCGGCCGCTGCAGGCT

Revision as of 20:00, 16 October 2014

Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity

Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in Bacillus subtilis in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time.

Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]