Difference between revisions of "Part:BBa K1351019"
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<partinfo>BBa_K1351019 short</partinfo> | <partinfo>BBa_K1351019 short</partinfo> | ||
− | Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br> | + | Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br> |
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br> | Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br> | ||
Suffix: TACTAGTAGCGGCCGCTGCAGGCT | Suffix: TACTAGTAGCGGCCGCTGCAGGCT |
Revision as of 20:00, 16 October 2014
Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity
Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in Bacillus subtilis in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time.
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]