Difference between revisions of "Part:BBa K1470004:Design"

Latest revision as of 19:55, 16 October 2014

Secreted Alkaline Phosphatase (SEAP)

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 209
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 520
Illegal NgoMIV site found at 1489
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 850
Illegal BsaI.rc site found at 1339

Design Notes

Design

Source

AG Weber Institute of Biology II / BIOSS Center for Biological Signalling Studies Albert-Ludwigs-Universität Freiburg Germany

References

[X] Maxam AM, Gilbert W (1980). „Sequencing end-labeled DNA with base-specific chemical cleavages“. Meth. Enzymol. Methods in Enzymology 65 (1): 499–560. [Y] Lowe ME. „Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein“. J Cell Biol. 1992 Feb;116(3):799-807.

@@ Line 1: / Line 1: @@
-blabla
+__NOTOC__
+<partinfo>BBa_K1470004 short</partinfo>
+<partinfo>BBa_K1470004 SequenceAndFeatures</partinfo>
+===Design Notes===
+Design
+===Source===
+AG Weber
+Institute of Biology II / BIOSS Center for Biological Signalling Studies
+Albert-Ludwigs-Universität Freiburg
+Germany
+===References===
+<p>
+[X] Maxam AM, Gilbert W (1980). „Sequencing end-labeled DNA with base-specific chemical cleavages“. Meth. Enzymol. Methods in Enzymology 65 (1): 499–560. [Y] Lowe ME. „Site-specific mutations in the COOH-terminus of placental alkaline phosphatase: a single amino acid change converts a phosphatidylinositol-glycan-anchored protein to a secreted protein“. J Cell Biol. 1992 Feb;116(3):799-807.
+</p>