Difference between revisions of "Part:BBa C0083:Experience"

Revision as of 19:11, 16 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0083

User Reviews

UNIQ4f72ccd5a262b057-partinfo-00000000-QINU

•••

iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.

UNIQ4f72ccd5a262b057-partinfo-00000003-QINU

Review number not understood iGEM Nagahama_2014

We removed LVA tag that renders protein susceptible to the action of tail-specific proteases(BBa_K1342002). And we analysed aspartic acid synthesized by E. coliJM109/BBa_K1342001.

UNIQ4f72ccd5a262b057-partinfo-00000005-QINU

@@ Line 35: / Line 35: @@
 |width='60%' valign='top'|
-We removed LVA tag that renders protein susceptible to the action of tail-specific proteases([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342002 BBa_K1342002]). And we analysed aspartic acid synthesized by <i>E. coli</i>.
+We removed LVA tag that renders protein susceptible to the action of tail-specific proteases([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342002 BBa_K1342002]). And we analysed aspartic acid synthesized by <i>
+E. coli</i>JM109/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342001 BBa_K1342001].
 |}
 ;
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K190019 EndReviews</partinfo>