Difference between revisions of "Part:BBa K1342003:Design"

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===Design Notes===
 
===Design Notes===
  
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One silent mutation was introduced to eliminate PstI restriction enzyme site and was eliminated at bases 588 and 657 with C → T mutation.
+
One silent mutation was introduced to eliminate PstI restriction enzyme site and was eliminated at bases 96 with C → T mutation.
  
 
===Source===
 
===Source===

Revision as of 17:57, 16 October 2014


SmtA Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One silent mutation was introduced to eliminate PstI restriction enzyme site and was eliminated at bases 96 with C → T mutation.

Source

Synechococcus sp. PCC7942

References

Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by introducing exogenic metallothionein gene, Koji Sode et al., J Mar Biotechnol (1998), 6:174-177