Difference between revisions of "Part:BBa K1431413:Design"

 
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===Design Notes===
 
===Design Notes===
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we will complete this part later
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After we use design the gRNA of HBV-2(BBa_K1431403), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .
 +
 
 +
But there are two problem:
 +
 
 +
First,the DSB of binding sequence is hard to observe in the cell;
 +
 
 +
Second,the whole HBV genome have a potential dangers in the lab.
 +
 
 +
So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of HB V to the change of reporter.
 +
The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.
  
  

Revision as of 17:54, 16 October 2014

target sequence2 for HBV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After we use design the gRNA of HBV-2(BBa_K1431403), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .

But there are two problem:

First,the DSB of binding sequence is hard to observe in the cell;

Second,the whole HBV genome have a potential dangers in the lab.

So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of HB V to the change of reporter. The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.


Source

we will complete this part later

References