Difference between revisions of "Part:BBa K1378003"
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=='''Usage and Biology'''==
 
=='''Usage and Biology'''==
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<p>MVN is a lectin isolated from the cyanobacteria <i>Microcystis aeruginosa</i> PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues <sup>[1]</sup>. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of <i>M. aeruginosa</i> PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in <i>E. coli</i> and its binding effect to cyanobacteria cells is species-specific <sup>[1]</sup>. </p>
  
 
<figure><img src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png"  
 
<figure><img src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png"  
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/><figcaption><b>Figure 2.</b> Solution structure of MVN. MVN consists of 2 domains, domain A  
 
/><figcaption><b>Figure 2.</b> Solution structure of MVN. MVN consists of 2 domains, domain A  
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and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is  
 
and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is  
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formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from  
 
formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from  
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Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>
 
Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>
  

Revision as of 16:46, 16 October 2014

Microvirin (MVN)---a lectin from Microcystis aeruginosa

Introduction

This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Microcystis aeruginosa PCC7806 specifically.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1029

Usage and Biology

MVN is a lectin isolated from the cyanobacteria Microcystis aeruginosa PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues [1]. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of M. aeruginosa PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in E. coli and its binding effect to cyanobacteria cells is species-specific [1].

<figure><img src="Peking2014ZZJ_MVN.png" /><figcaption>Figure 2. Solution structure of MVN. MVN consists of 2 domains, domain A and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>