Difference between revisions of "Part:BBa K1378003"

Revision as of 16:42, 16 October 2014

INPNC-linker2-MVN-linker1-mRFP

Introduction

This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Microcystis aeruginosa PCC7806 specifically.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 466
Illegal AgeI site found at 1915
Illegal AgeI site found at 2027
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1029

Usage and Biology

<figure><img src=""

/><figcaption>Figure 2. Solution structure of MVN. MVN consists of 2 domains, domain A

and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is

formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from

Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K1378003 short</partinfo>
+<partinfo>BBa_K1378005 short</partinfo>
-<p>This plasmid is a basic part of the mannan binding lectin <b>Microvirin (MVN)</b>. Microvirin can be expressed in <i>E. coli</i> and it can bind to <i>Micorcystis aeruginosa</i> PCC7806 specifically.
+=='''Introduction'''==
-<!-- Add more about the biology of this part here
+This plasmid is a basic part of the mannan binding lectin <b>Microvirin (MVN)</b>. Microvirin can be expressed in <i>E. coli</i> and it can bind to <i>Microcystis aeruginosa</i> PCC7806 specifically.
-===Usage and Biology===
-<p>MVN is a lectin isolated from the cyanobacteria <i>Microcystis aeruginosa</i> PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues <sup>[1]</sup>. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of <i>M. aeruginosa</i> PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in <i>E. coli</i> and its binding effect to cyanobacteria cells is species-specific <sup>[1]</sup>.
-<figure><img src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png"/><figcaption><b>Figure 1.</b> Solution structure of MVN. MVN consists of 2 domains, domain A and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K1378003 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K1378005 SequenceAndFeatures</partinfo>
+=='''Usage and Biology'''==
+<figure><img src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png"
+/><figcaption><b>Figure 2.</b> Solution structure of MVN. MVN consists of 2 domains, domain A
+and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is
+formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from
+Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1378003 parameters</partinfo>
+<partinfo>BBa_K1378005 parameters</partinfo>
 <!-- -->