Difference between revisions of "Part:BBa K1378003"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1378003 short</partinfo>
 
<partinfo>BBa_K1378003 short</partinfo>
  
−
microvirin(MVN),a lectin from M.aeruginosa,attributes to the colony formation of cyanobacterium. We display MVN on the surface of E.coli so that E.coli can bind to cyanobacterium.
+
<p>This plasmid is a basic part of the mannan binding lectin <b>Microvirin (MVN)</b>. Microvirin can be expressed in <i>E. coli</i> and it can bind to <i>Micorcystis aeruginosa</i> PCC7806 specifically.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 +
 
===Usage and Biology===
 
===Usage and Biology===
 +
<p>MVN is a lectin isolated from the cyanobacteria <i>Microcystis aeruginosa</i> PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues <sup>[1]</sup>. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of <i>M. aeruginosa</i> PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in <i>E. coli</i> and its binding effect to cyanobacteria cells is species-specific <sup>[1]</sup>.
  
 +
<figure><img src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png"/><figcaption><b>Figure 1.</b> Solution structure of MVN. MVN consists of 2 domains, domain A and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:40, 16 October 2014

Microvirin (MVN)---a lectin from Microcystis aeruginosa

This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Micorcystis aeruginosa PCC7806 specifically. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 23