Difference between revisions of "Part:BBa K1412089:Design"
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<partinfo>BBa_K1412089 short</partinfo> | <partinfo>BBa_K1412089 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | In our project, we consider different kinds of keys can open our lock.Based on our project, we design the part based on the two principle(2): | ||
+ | 1.The binding fore between taRNA and crRNA is stronger than that between crRNA and RBS or that between aptamer and taRNA. | ||
+ | |||
+ | 2.RBS and AUG are separated by about 6 bases. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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+ | 1.Callura J M, Cantor C R, Collins J J. Genetic switchboard for synthetic biology applications[J]. Proceedings of the National Academy of Sciences, 2012, 109(15): 5850-5855. | ||
+ | 2.Farren J Isaasc, Daniel J Dwyer, Ding C, Pervouchine DD, Cantor CR, Collins JJ, et al.Engineered riboregulators enable post-translational control of gene expression. Nat Biotechnol. 2004 Jul;22(7):841-7. |
Revision as of 15:36, 16 October 2014
Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our project, we consider different kinds of keys can open our lock.Based on our project, we design the part based on the two principle(2):
1.The binding fore between taRNA and crRNA is stronger than that between crRNA and RBS or that between aptamer and taRNA.
2.RBS and AUG are separated by about 6 bases.
Source
References
1.Callura J M, Cantor C R, Collins J J. Genetic switchboard for synthetic biology applications[J]. Proceedings of the National Academy of Sciences, 2012, 109(15): 5850-5855. 2.Farren J Isaasc, Daniel J Dwyer, Ding C, Pervouchine DD, Cantor CR, Collins JJ, et al.Engineered riboregulators enable post-translational control of gene expression. Nat Biotechnol. 2004 Jul;22(7):841-7.