Difference between revisions of "Part:BBa K1412088"
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<partinfo>BBa_K1412088 short</partinfo> | <partinfo>BBa_K1412088 short</partinfo> | ||
| − | + | this part was made from the riboregulator taRNA by Isaacs et al(1)and theophylline aptamer by Lynch et al(2).Based on our desigen, the sequence of taRNA '''change some bases''', while theophylline aptamer in our project is the same as Justin P.Gallivan group(2). | |
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| + | [[File:Aptamer_taRNA.jpg|500px]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Fabrication notes: | ||
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| + | In order to response difference molecular to regulate the gene circuit, we add theophylline aptamer based on the sequence of taRNA. When this piece is transcribed into mRNA,the aptamer's second structure complement with taRNA in term of the optimal configuration.When theophylline add into medium, aptamer can specificity combine it,changing the second structure,leading to the taRNA exposure.Now the key play a role to "wake up" our project lock. | ||
| + | Part is also available with a constitutively on promoter as BBa_R0040, which is promoter tetR. | ||
| + | |||
| + | Experimental Notes: | ||
| + | |||
| + | see our project. | ||
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Latest revision as of 15:14, 16 October 2014
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit
this part was made from the riboregulator taRNA by Isaacs et al(1)and theophylline aptamer by Lynch et al(2).Based on our desigen, the sequence of taRNA change some bases, while theophylline aptamer in our project is the same as Justin P.Gallivan group(2).
Usage and Biology
Fabrication notes:
In order to response difference molecular to regulate the gene circuit, we add theophylline aptamer based on the sequence of taRNA. When this piece is transcribed into mRNA,the aptamer's second structure complement with taRNA in term of the optimal configuration.When theophylline add into medium, aptamer can specificity combine it,changing the second structure,leading to the taRNA exposure.Now the key play a role to "wake up" our project lock. Part is also available with a constitutively on promoter as BBa_R0040, which is promoter tetR.
Experimental Notes:
see our project.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]