Difference between revisions of "Part:BBa K1412089"

Revision as of 14:53, 16 October 2014

Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit

This part is based on the Callra J M et al’s (1)riboregulator crRNA. In order to use our project "aptamer key" (BBa_B1412088)to open the lock, we use AGGAGGAAA as our RBS. Here we add BBa_B01060,which is promoter tetR to transcrip our DNA.

Usage and Biology

The Callra J M et al's work is the continuation of the work done by Isaacs et al(2). From the paper, we notice that we can add aptamer before taRNA to regulate taRNA express, which in turn influence the expression of the crRNA. In our project, we consider our lock can be opened by different RNA aptamer if the aptamer construct the taRNA in the same way according to our project.

When this part was transcript into mRNA, it forms a stem-loop structure which “locks” the ribosome binding site. This folded mRNA structure is known as the “ cis-repressed” part. If BBa_K1412088 available, when we add theophylline into the medium, the taRNA can exposes, which destabilize the stem-loop enough to allow the corresponding taRNA to interject crRNA and open the loop.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The Callra J M’ work is the continuation of the work done by Isaacs (2). From the paper, we notice that we can add aptamer before taRNA to regulate taRNA express, which in turn influence the expression of the crRNA. In our project, we consider our lock can be opened by different RNA aptamer if the aptamer construct the taRNA in the same way according to our project.
+The Callra J M et al's work is the continuation of the work done by Isaacs et al(2). From the paper, we notice that we can add aptamer before taRNA to regulate taRNA express, which in turn influence the expression of the crRNA. In our project, we consider our lock can be opened by different RNA aptamer if the aptamer construct the taRNA in the same way according to our project.
 When this part was transcript into mRNA, it forms a stem-loop structure which “locks” the ribosome binding site. This folded mRNA structure is known as the “ cis-repressed” part. If BBa_K1412088 available, when we add theophylline into the medium, the taRNA can exposes, which destabilize the stem-loop enough to allow the corresponding taRNA to interject crRNA and open the loop.