Difference between revisions of "Part:BBa K1381014:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1381014 short</partinfo>
 
<partinfo>BBa_K1381014 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Designed in three length version 10 bases BBa_K1381014 , 15 bases BBa_K1381015 and 20 bases BBa_K1381016
+
This sRNA has an antisense region of ten nucleotides. We also constructed two other sRNA that has an antisense region specific for USP45. The difference is the length of the antisense region. One has an antisense region of [https://parts.igem.org/Part:BBa_K1381015 15] nucleotides and the other has one with [https://parts.igem.org/Part:BBa_K1381016 20] nucleotides. Usually there is a trade of in specificity and strength with varying length of antisense region. And that’s the reason we have three different sRNA. These three parts should work in theory but have not been sufficiently tested and the relative strength of the three parts is unknown. But we know that it doesn’t bind to any vital gene in <i>e.coli</i> and silence that because it was possible to express this silencing sRNA.
−
 
+
  
  

Revision as of 14:23, 16 October 2014

Silencing sRNA (10 bases long) for USP45 (Based on Spot42)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sRNA has an antisense region of ten nucleotides. We also constructed two other sRNA that has an antisense region specific for USP45. The difference is the length of the antisense region. One has an antisense region of 15 nucleotides and the other has one with 20 nucleotides. Usually there is a trade of in specificity and strength with varying length of antisense region. And that’s the reason we have three different sRNA. These three parts should work in theory but have not been sufficiently tested and the relative strength of the three parts is unknown. But we know that it doesn’t bind to any vital gene in e.coli and silence that because it was possible to express this silencing sRNA.


Source

BBa_K864440

References