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| − | <I>Wanglu</I>
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| − | We also met some problem in our construction. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. We just ligated the tetR protein coding sequence with lac promoter, and repeated for many times. However, the sequencing result suggested that there was always either a deletion of the promoter or an unknown insertion in tetR coding sequence in our samples. We speculate that tetR protein has cytotoxicity and burden the E.coli, so the samples were always with mutations that cause of no expression of tetR protein or its inactivation.
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Revision as of 14:07, 16 October 2014
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UNIQfe8a515a06984546-partinfo-00000000-QINU
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••••
Aberdeen_Scotland 2009
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The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked as expected.
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