Difference between revisions of "Part:BBa K1475007:Design"

 
 
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<partinfo>BBa_K1475007 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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This part was constructed using A3 standard assembly from Pcon-LacI-term  ([https://parts.igem.org/Part:BBa_K1474008 Part:BBa_ K1474008]), the
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lacI promoter ([https://parts.igem.org/Part:BBa_R0010 Part:BBa_R0010]) and the GFP generator ([https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840]).
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<br>
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<br>
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The sequence of the lacI repressor is from the repressilator of Elowitz and Leibler (2000) [1]. The sequence is modified from the wild-type by using ATG as start codon instead of GTG.
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<br>
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<br>
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The GFP used has some mutations from wtGFP, for more information see the basic part entry page [https://parts.igem.org/Part:BBa_E0040:Design  Part:BBa_E0040:Design].
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===Source===
 
===Source===
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Represillator of Elowitz and Leibler (2000). [1]
  
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===References===
 
===References===
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1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
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<br>
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UniProt accesstion for ''Escherichia coli'' lacI: http://www.uniprot.org/uniprot/P03023
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Entrez gene accession for ''Escherichia coli'' lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
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<br>
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Protein database entry for ''Escherichia coli'' lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

Latest revision as of 12:49, 16 October 2014

GFP controlled by lacI and plac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2057


Design Notes

This part was constructed using A3 standard assembly from Pcon-LacI-term (Part:BBa_ K1474008), the lacI promoter (Part:BBa_R0010) and the GFP generator (Part:BBa_E0840).

The sequence of the lacI repressor is from the repressilator of Elowitz and Leibler (2000) [1]. The sequence is modified from the wild-type by using ATG as start codon instead of GTG.

The GFP used has some mutations from wtGFP, for more information see the basic part entry page Part:BBa_E0040:Design.



Source

Represillator of Elowitz and Leibler (2000). [1]


References

1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG