Difference between revisions of "Part:BBa K1475006"

Revision as of 12:33, 16 October 2014

GFP controlled by TetR+LVA and pTet.

This composite part has GFP controlled by a TetR, p(TetR) expression system. The TetR repressor protein binds to the Tet promoter repressing expression. [1] The tet repressor protein is inhibited by [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] or the analogue dox (doxycycline) hereby inducing the expression of GFP.[2]

Please note that this part contains the TetR repressor with LVA rapid degradation tag, for a similar part containing TetR repressor without LVA tag please see Part:BBa_K1475005. The TetR without LVA tag can be found here as a basic part: [https://parts.igem.org/Part:BBa_ K1475003

Part:BBa_ K1475003].

Usage and Biology

This part can be used to test the expression from the TetR p(TetR) system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1485

References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
3. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1475006 short</partinfo>
-This composite part has GFP controlled by a TetR+LVA pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
+This composite part has GFP controlled by a TetR, p(TetR) expression system. The TetR repressor protein binds to the Tet promoter repressing expression. [1] The tet repressor protein is inhibited by [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] or the analogue dox (doxycycline) hereby inducing the expression of GFP.[2]
+<br><br>
+Please note that this part contains the TetR repressor with LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag], for a similar part containing TetR repressor without LVA tag please see [https://parts.igem.org/Part:BBa_K1475005 Part:BBa_K1475005]. The TetR without LVA tag can be found here as a basic part: [https://parts.igem.org/Part:BBa_ K1475003
+ Part:BBa_ K1475003].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part can be used to test the expression from the TetR p(TetR) system.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1475006 SequenceAndFeatures</partinfo>
@@ Line 17: / Line 20: @@
 <partinfo>BBa_K1475006 parameters</partinfo>
 <!-- -->
+===References===
+. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
+<br>
+. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
+<br>
+. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850