Difference between revisions of "Part:BBa K1475003:Design"
(4 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part: | + | The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040]) using the following primers, which are designed to remove the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag]: |
<br> | <br> | ||
− | Forward primer: | + | Forward primer (containing prefix): |
<br> | <br> | ||
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG | AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG | ||
<br> | <br> | ||
− | Reverse primer: | + | Reverse primer (containing suffix): |
<br> | <br> | ||
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC | TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC | ||
− | |||
− | |||
− | |||
===Source=== | ===Source=== | ||
Line 25: | Line 22: | ||
===References=== | ===References=== | ||
− | 1 | + | 1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677 |
− | + | <br> | |
+ | 2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components |
Latest revision as of 12:16, 16 October 2014
tetracyclin repressor from transposon Tn10
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers, which are designed to remove the LVA rapid degradation tag:
Forward primer (containing prefix):
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer (containing suffix):
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC
Source
Organizm: Escherichia coli
References
1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components