Difference between revisions of "Part:BBa K1475003"
 
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<partinfo>BBa_K1475003 short</partinfo>
 
<partinfo>BBa_K1475003 short</partinfo>
  
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This part contains the coding sequence of the TetR protein without the LVA tag. TetR binds to the pTet promoter and represses transcription. aTc (anhydrotetracycline) bind to the TetR protein and inhibits the function of TetR.
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The coding region of the frequently used tetracycline repressor (TetR) [https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040] modified by removing the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag], this will in theory serve to increase the protein half-life and in practice increase the concentration of the protein in the cell. This TetR is the tTA subtype which on binding to the p(TetR) promoter [https://parts.igem.org/Part:BBa_R0040 Part:BBa_R0040] blocks transcription. [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] binds to the Tet repressor inhibiting its function and hereby releasing the repression on the Tet promoter.
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===Usage and Biology===
 
===Usage and Biology===
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In addition to ATc (anhydrotetracycline), dox (Doxycycline) can also be used as inducer. [2]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1475003 parameters</partinfo>
 
<partinfo>BBa_K1475003 parameters</partinfo>
 
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===References===
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1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
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<br>
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2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components

Latest revision as of 12:10, 16 October 2014

tetracyclin repressor from transposon Tn10

The coding region of the frequently used tetracycline repressor (TetR) Part:BBa_C0040 modified by removing the LVA rapid degradation tag, this will in theory serve to increase the protein half-life and in practice increase the concentration of the protein in the cell. This TetR is the tTA subtype which on binding to the p(TetR) promoter Part:BBa_R0040 blocks transcription. [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] binds to the Tet repressor inhibiting its function and hereby releasing the repression on the Tet promoter.


Usage and Biology

In addition to ATc (anhydrotetracycline), dox (Doxycycline) can also be used as inducer. [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components