Difference between revisions of "Part:BBa K1381025"
 
Line 4: Line 4:
 
Used for N-terminal-BFP fusion protein using Freiburg standard. Contains an RBS and ATG before the NgoMIV site so can be expressed separetely if needed.
 
Used for N-terminal-BFP fusion protein using Freiburg standard. Contains an RBS and ATG before the NgoMIV site so can be expressed separetely if needed.
  
−
This part is a mutagenise of BFP [https://parts.igem.org/Part:BBa_K592024 BBa_K592024] to make it compatible with RFC25. It was designed to get an ATG followed by an NgoMIV site upstream of the BFP CDS. The original ATG from the BFP CDS was removed.  
+
This part is a mutagenise of [https://parts.igem.org/Part:BBa_K592024 BBa_K592024] (B0034-BFP) to make it compatible with RFC25. It was designed to get an ATG followed by an NgoMIV site upstream of the BFP CDS. The original ATG from the BFP CDS was removed.  
  
  

Latest revision as of 10:51, 16 October 2014

B0034-BFP (RFC25)

Used for N-terminal-BFP fusion protein using Freiburg standard. Contains an RBS and ATG before the NgoMIV site so can be expressed separetely if needed.

This part is a mutagenise of BBa_K592024 (B0034-BFP) to make it compatible with RFC25. It was designed to get an ATG followed by an NgoMIV site upstream of the BFP CDS. The original ATG from the BFP CDS was removed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]