Difference between revisions of "Part:BBa K1317003:Design"

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The design and in-silico cloning has been performed on Genome Compiler
 
The design and in-silico cloning has been performed on Genome Compiler
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Consensus sequence:
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In the literature we found out that the natural elastin has some repetitions, in particular the sequence VPGXG seems to be repeated. X is V, L, or A, and it represents 9% of the whole polypeptide. This particular sequence were translated with codon optimization for ''E.coli'' to produce a (VPGXG)x protein. Elastin properties were checked to see if they are still existing after cloning into bacteria to produce the recombinant protein named elastin like polypeptide (ELP).
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===Source===
 
===Source===
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===References===
 
===References===
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Doreen M. Floss et al. ''ELASTIN-like polypeptides revolutionize recombinant protein expression and their biomedical application.'' Trends in Biotechnology Vol.28 No.1 (PMID 19897265)

Revision as of 10:29, 16 October 2014

CDS for Elastin like polypeptide (ELP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene has been assembled by using overlapping oligos and Gibson assembly. The restriction site NheI has been added to get rid of the STOP codon. It is an improvement in the biobricks system, because it can be used to reassemble the coding sequence. For example in our project, another copy of the gene can be added just after the first one, so influence of the length of the final polymer on mechanical properties can be determined.

The design and in-silico cloning has been performed on Genome Compiler

Consensus sequence:

In the literature we found out that the natural elastin has some repetitions, in particular the sequence VPGXG seems to be repeated. X is V, L, or A, and it represents 9% of the whole polypeptide. This particular sequence were translated with codon optimization for E.coli to produce a (VPGXG)x protein. Elastin properties were checked to see if they are still existing after cloning into bacteria to produce the recombinant protein named elastin like polypeptide (ELP).


Source

Next

References

Doreen M. Floss et al. ELASTIN-like polypeptides revolutionize recombinant protein expression and their biomedical application. Trends in Biotechnology Vol.28 No.1 (PMID 19897265)