Difference between revisions of "Part:BBa K515102:Experience"
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The plants root exudates contain TCA intermediates that can attract bacteria having the ability of chemotaxis. <i>E.coli</i> has five kinds of chemoreceptors, which interact with factors of the flagella that leads to <a href="#chemo">chemotaxis</a>. But <i>E.coli</i> doesn’t have specific chemotaxis towards some TCA intermediates while Pseudomonas putida has some McpS, like McfQ and McfR. We made a part <a href="#top">BBa-</a> containing the sequence of McfR, which detects succinate, malate and fumarate. Then we detected its chemotaxis towards malate and succinate, and did the same assay to BBa_K515102. | The plants root exudates contain TCA intermediates that can attract bacteria having the ability of chemotaxis. <i>E.coli</i> has five kinds of chemoreceptors, which interact with factors of the flagella that leads to <a href="#chemo">chemotaxis</a>. But <i>E.coli</i> doesn’t have specific chemotaxis towards some TCA intermediates while Pseudomonas putida has some McpS, like McfQ and McfR. We made a part <a href="#top">BBa-</a> containing the sequence of McfR, which detects succinate, malate and fumarate. Then we detected its chemotaxis towards malate and succinate, and did the same assay to BBa_K515102. | ||
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Revision as of 02:53, 16 October 2014
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The plants root exudates contain TCA intermediates that can attract bacteria having the ability of chemotaxis. E.coli has five kinds of chemoreceptors, which interact with factors of the flagella that leads to chemotaxis. But E.coli doesn’t have specific chemotaxis towards some TCA intermediates while Pseudomonas putida has some McpS, like McfQ and McfR. We made a part BBa- containing the sequence of McfR, which detects succinate, malate and fumarate. Then we detected its chemotaxis towards malate and succinate, and did the same assay to BBa_K515102. |
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