Difference between revisions of "Part:BBa K1321014:Design"
(Created page with "__NOTOC__ <partinfo>BBa_K1321014 short</partinfo> <partinfo>BBa_K1321014 SequenceAndFeatures</partinfo> ===Design Notes=== Nucleotide sequence was first obtained from the rel...") |
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Latest revision as of 02:43, 16 October 2014
CBDCipA with N and C-terminal linker
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 148
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 148
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 148
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 148
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 148
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Nucleotide sequence was first obtained from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 then codon optimised for E. coli. The DNA sequence for RFC25 prefix and suffix was appeneded to the sequence, with an additional 4 basepairs (gatc) at the beginning and end of the sequence to allow for space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
Source
The designed construct was ordered from as a GeneArt® String (Invitrogen™ Life Technologies).