Difference between revisions of "Part:BBa K1462150:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1462150 short</partinfo> | <partinfo>BBa_K1462150 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of AdhE2 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. | |
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===Source=== | ===Source=== | ||
| − | + | AdhE2 is from Clostridium beijerinckii,the other elements is provided by official. | |
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===References=== | ===References=== | ||
| + | Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36. | ||
Revision as of 02:23, 16 October 2014
Gal1+AdhE2+GFP+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3782
Design Notes
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of AdhE2 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Source
AdhE2 is from Clostridium beijerinckii,the other elements is provided by official.
References
Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.