Difference between revisions of "Part:BBa K1080012"

Revision as of 00:47, 16 October 2014

DVR1

3,8-divinyl protochlorophyllidea 8-vinyl reductase - Predicted chloroplast transit peptide 1-58; [PMID: 15695432; 15849308] This enzyme reduces divinyl protochlorophyllide a to monovinyl protochlorophyllide a. This reduction is essential for chlorophyllide a synthesis, but can probably occur at any stage between protoporphyrin IX and chlorophyllide a. [1, 2]

Usage and Biology

Figure 1: Reaction catalysed by DVR1. [3]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 288
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Amino acid sequence

MAMAASRQAV RVAAAVDADY RKREPKDVRV LVVGPTGYIG KFVVKELVSR GYNVVAFARE
NAGIKGKMGR EDIVKEFHGA EVRFGSVLDP ASLRDVAFKD PVDVVVSCLA SRTGGKKDSW
LIDYTATKNS LDVARASGAK HFVLLSAICV QKPLLEFQKA KLQFESDLQA AGDITYSIVR
PTAFFKSIAG QIDIVKKGNP YVMFGDGNLA ACKPISEADL ASFIADCVTE QNKVNKVLPI
GGPSKAFTAK QQADLLFNIT GLPPKYFPVP VALMDGMIGL FDSLAKLFPQ LEDSAEFARI
GKYYATESML VYDEARGVYR KTKRLVTART RWKTSSLVQ

References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 339

Molecular weight: 37034.9

Theoretical pI: 9.40

Amino acid composition:
Ala (A) 40 11.8%
Arg (R) 19 5.6%
Asn (N) 8 2.4%
Asp (D) 22 6.5%
Cys (C) 4 1.2%
Gln (Q) 11 3.2%
Glu (E) 14 4.1%
Gly (G) 24 7.1%
His (H) 2 0.6%
Ile (I) 16 4.7%
Leu (L) 27 8.0%
Lys (K) 29 8.6%
Met (M) 7 2.1%
Phe (F) 18 5.3%
Pro (P) 15 4.4%
Ser (S) 21 6.2%
Thr (T) 14 4.1%
Trp (W) 2 0.6%
Tyr (Y) 11 3.2%
Val (V) 35 10.3%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 36 Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon C 1670 Hydrogen H 2668 Nitrogen N 450 Oxygen O 477 Sulfur S 11

Formula: C1670H2668N450O477S11 Total number of atoms: 5276

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 27640 Abs 0.1% (=1 g/l) 0.746, assuming all pairs of Cys residues form cystines

Ext. coefficient 27390 Abs 0.1% (=1 g/l) 0.740, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is:

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 31.02 This classifies the protein as stable.

Aliphatic index: 91.21

Grand average of hydropathicity (GRAVY): -0.019

Source

Chlamydomonas reinhardtii,

References

1. Willows, R.D., Chlorophylls, in Plant Pigments and their Manipulation, K. Davies, Editor. 2004, Blackwell Publishing.
2. Willows, R.D., Chlorophylls, in Encyclopedia of plant and crop science. 2004.
3. DiatomCyc. Chlamydomonas reinhardtii Reaction: 1.3.1.75. Available from: http://akongo.psb.ugent.be/CHLAMY/NEW-IMAGE?type=REACTION&object=RXN1F-72.

@@ Line 112: / Line 112: @@
 ===References===
+.	Willows, R.D., ''Chlorophylls'', in ''Plant Pigments and their Manipulation'', K. Davies, Editor. 2004, Blackwell Publishing.
+<br>2.	Willows, R.D., ''Chlorophylls'', in ''Encyclopedia of plant and crop science''. 2004.
+<br>3.	DiatomCyc. ''Chlamydomonas reinhardtii Reaction: 1.3.1.75''. Available from: http://akongo.psb.ugent.be/CHLAMY/NEW-IMAGE?type=REACTION&object=RXN1F-72.
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