Difference between revisions of "Part:BBa K1442111"
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− | == DPP-IV == | + | == DPP-IV 3'UTR (target of the siRNA)== |
− | As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113). | + | <p>As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113). </p> |
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== Usage == | == Usage == | ||
+ | <p>This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.</p> | ||
− | + | == Model == | |
+ | <p><html><body><img src="https://static.igem.org/mediawiki/parts/e/ec/DR_dppiv_model.PNG"/></body></html></p> | ||
+ | <p><html><body><img src="https://static.igem.org/mediawiki/parts/2/23/Rds_dppiv_model.PNG"/></body></html></p> | ||
+ | <p><html><body><img src="https://static.igem.org/mediawiki/parts/8/84/DT_dppiv_model.PNG"/></body></html></p> | ||
+ | <p><html><body><img src="https://static.igem.org/mediawiki/parts/f/fd/DC_dppiv_model.PNG"/></body></html></p> | ||
+ | <p><html><body><img src="https://static.igem.org/mediawiki/parts/9/95/DG_dppiv_model.PNG"/></body></html></p> | ||
+ | <p>Where R<sub>i</sub>=concentration of interfering RNA</p> | ||
+ | <p>T=concentration of target RNA</p> | ||
+ | <p>R<sub>ds</sub>=concentration of double stranded RNA (made from interfering RNA and target association)</p> | ||
+ | <p>C=siRNA/RISC complex</p> | ||
+ | <p>and G=GFP expression</p> |
Latest revision as of 23:24, 15 October 2014
DPP-IV (our siRNA target)
DPP-IV 3'UTR (target of the siRNA)
As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).
The schematic above shows the organisation of the DPP-IV target
Usage
This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.
Model
Where Ri=concentration of interfering RNA
T=concentration of target RNA
Rds=concentration of double stranded RNA (made from interfering RNA and target association)
C=siRNA/RISC complex
and G=GFP expression