Difference between revisions of "Part:BBa K1554003"
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EaDAcT is a diacylglycerol acetyltransferase from the plant ''Euonymus alatus''.
 
EaDAcT is a diacylglycerol acetyltransferase from the plant ''Euonymus alatus''.
  
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===Usage and Biology===
 
===Usage and Biology===
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The EaDAcT protein is a diacylglycerol acetyltransferase coming from ''Euonymus alatus'' which transforms fatty alcohols into fatty aldehides. Our team used it in with [https://parts.igem.org/Part:BBa_K1554001 Part:BBa_K1554001] and [https://parts.igem.org/Part:BBa_K1554002 Part:BBa_K1554002] to produce the insect pheromone Z11-16:OAc in ''Nicotiana benthamiana'' using palmitate:CoA as precursor. Its role is to convert Z11-16:OH into Z11-16:OAc.
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When [https://parts.igem.org/Part:BBa_K1554001 Part:BBa_K1554001]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1554003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1554003 SequenceAndFeatures</partinfo>

Revision as of 22:53, 15 October 2014

EaDAcT

EaDAcT is a diacylglycerol acetyltransferase from the plant Euonymus alatus.


Usage and Biology

The EaDAcT protein is a diacylglycerol acetyltransferase coming from Euonymus alatus which transforms fatty alcohols into fatty aldehides. Our team used it in with Part:BBa_K1554001 and Part:BBa_K1554002 to produce the insect pheromone Z11-16:OAc in Nicotiana benthamiana using palmitate:CoA as precursor. Its role is to convert Z11-16:OH into Z11-16:OAc.

When Part:BBa_K1554001


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]