Difference between revisions of "Part:BBa K1399014"

Latest revision as of 22:39, 15 October 2014

Plac-GFP(AAV)

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of green fluorescence and promoter activity.

Figure 1 SsrA degron mediated protein degradation. (A) Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). (B) The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 870

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1399014 short</partinfo>
 Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
-The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
+The tagged GFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of green fluorescence and promoter activity.
+[[File:EDiGEM14-SsrA degron mediated degradation.png|400px|thumb|center|'''Figure 1 SsrA degron mediated protein degradation.''' ('''A''') Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). ('''B''') The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.]]
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