Difference between revisions of "Part:BBa K1399010"
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Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
 
Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
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The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity.
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The tagged RFP is actively degraded within cell (Figure 1), thus it provides better temporal resolution of red fluorescence and promoter activity.
  
 +
[[File:EDiGEM14-SsrA degron mediated degradation.png|400px|thumb|center|'''Figure 1 SsrA degron mediated protein degradation.''' ('''A''') Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). ('''B''') The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.]]
  
  

Revision as of 22:26, 15 October 2014

Plac-RFP(AAV)

Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell (Figure 1), thus it provides better temporal resolution of red fluorescence and promoter activity.

Figure 1 SsrA degron mediated protein degradation. (A) Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). (B) The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]