Difference between revisions of "Part:BBa K1475002"

 
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<partinfo>BBa_K1475002 short</partinfo>
 
<partinfo>BBa_K1475002 short</partinfo>
  
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This part encodes the FAT-2 delta 12 desaturase from C. elegans and uses oleic acid (C18:1&#916;9) as a substrate and produces linoleic acid (C18:2&#8710;9,12). FAT-2 has also been reported to be able to desaturate fatty acids of lengths C14 to C18 (Xue-Rong Zhou et al., 2011).
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This part encodes the FAT-2 delta 12 desaturase from ''Caenorhabditis elegans'' and uses oleic acid (C18:1&#916;9) as a substrate to produce linoleic acid (C18:2&#8710;9,12).  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Fatty acyl desaturases act on acyl groups to introduce double bonds. Fat-2 is a ∆12 fatty acyl desaturase which is able to introduce a double bond in C16 and C18 fatty acids in Caenorhabditis elegans, and it has been shown to work as a ∆15 desaturase in yeast using oleic acid (C18:1∆9) as a substrate [1]. In nature the desaturation of fatty acids is important for keeping the cell membrane fluid in cold conditions [2].
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This part contains only the coding region of the fat-2 ∆12-desaturase from “Caenorhabditis elegans”, the natural gene consists of three exons. [2]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475002 SequenceAndFeatures</partinfo>

Revision as of 21:25, 15 October 2014

FAT-2 delta 12 desaturase from C. elegans

This part encodes the FAT-2 delta 12 desaturase from Caenorhabditis elegans and uses oleic acid (C18:1Δ9) as a substrate to produce linoleic acid (C18:2∆9,12).


Usage and Biology

Fatty acyl desaturases act on acyl groups to introduce double bonds. Fat-2 is a ∆12 fatty acyl desaturase which is able to introduce a double bond in C16 and C18 fatty acids in Caenorhabditis elegans, and it has been shown to work as a ∆15 desaturase in yeast using oleic acid (C18:1∆9) as a substrate [1]. In nature the desaturation of fatty acids is important for keeping the cell membrane fluid in cold conditions [2].

This part contains only the coding region of the fat-2 ∆12-desaturase from “Caenorhabditis elegans”, the natural gene consists of three exons. [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
    Illegal BamHI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 169
    Illegal SapI site found at 582