Difference between revisions of "Part:BBa K1475010:Design"
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===Design Notes=== | ===Design Notes=== | ||
The part was constructed using standard assembly from: [https://parts.igem.org/Part:BBa_K1475003 Part: BBa_K1475003] and [https://parts.igem.org/Part:BBa_K118024 Part:BBa_K118024] | The part was constructed using standard assembly from: [https://parts.igem.org/Part:BBa_K1475003 Part: BBa_K1475003] and [https://parts.igem.org/Part:BBa_K118024 Part:BBa_K118024] | ||
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===Source=== | ===Source=== | ||
| − | Dxs: Escherichia coli | + | Dxs: ''Escherichia coli'' |
<br> | <br> | ||
| − | LIMS1: Citrus lemon | + | LIMS1: ''Citrus lemon'' |
<br> | <br> | ||
| − | appY: Escherichia coli | + | appY: ''Escherichia coli'' |
===References=== | ===References=== | ||
Latest revision as of 21:13, 15 October 2014
dxs+LIMS1+appY regulated by the tet promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 808
Illegal SapI.rc site found at 2068
Design Notes
The part was constructed using standard assembly from: Part: BBa_K1475003 and Part:BBa_K118024
Source
Dxs: Escherichia coli
LIMS1: Citrus lemon
appY: Escherichia coli
References
1. Leighanne A. Brammer et al.: 1-Deoxy-d-xylulose 5-Phosphate Synthase Catalyzes a Novel Random Sequential Mechanism. J Biol Chem. Oct 21, 2011; 286(42): p. 36522–36531. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196101/
2. BRENDA, 2014: EC 2.2.1.7 - 1-deoxy-D-xylulose-5-phosphate synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=2.2.1.7&Suchword=&organism%5B%5D=Escherichia+coli&show_tm=0
3. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0
4. UniProt, P05052- APPY_ECOLI, http://www.uniprot.org/uniprot/P05052