Difference between revisions of "Part:BBa K1342002"
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===TLC Assay===
 
===TLC Assay===
  
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[[File:TLC_20140929_.jpg|200px|thumb|left|Analysis of aspartate from strain E.coli K-12/[https://parts.igem.org/Part:BBa_K1342001 BBa_K1342001] by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)]]
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[[File:TLC_20140929_.jpg|200px|thumb|left|
 +
fig.1
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<br>
 +
Analysis of aspartate from strain E.coli K-12/[https://parts.igem.org/Part:BBa_K1342001 BBa_K1342001] by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)]]
  
  
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[[File:TLC_intensity_of_the_band.jpg|400px|thumb|center|intensity of the band which is the Quantitative data.]]
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[[File:TLC_intensity_of_the_band.jpg|400px|thumb|center|
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fig.2
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<br>
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intensity of the band which is the Quantitative data of the aspartic acid synthesis.]]

Revision as of 19:35, 15 October 2014

AspA LVA(-)

Asp PDB.jpg
We removed LVA tag that renders protein susceptible to the action of tail-specific proteases from BBa_C0083 .
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 964
    Illegal AgeI site found at 493
    Illegal AgeI site found at 1345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1004



TLC Assay

fig.1
Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)


fig.2
intensity of the band which is the Quantitative data of the aspartic acid synthesis.