Difference between revisions of "Part:BBa K1554005:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1554005 short</partinfo>
 
<partinfo>BBa_K1554005 short</partinfo>
  
 
<partinfo>BBa_K1554005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1554005 SequenceAndFeatures</partinfo>
−
 
  
 
===Design Notes===
 
===Design Notes===
−
The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.  
+
The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 17: Line 13:
 
Barnase [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173]
 
Barnase [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173]
  
−
AmilGFP [Waiting for Norwich team part name in registry]
+
AmilCP [https://parts.igem.org/Part:BBa_J72173 Part:BBa_K1467202]
  
 
P35s [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
 
P35s [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
 +
 +
T35s: GoldenBraid 2.0 Collection
  
 
===References===
 
===References===

Revision as of 19:07, 15 October 2014

Blue biosafety module for plants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3097
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.

Source

TA29 promoter Part:BBa_K1554000

Barnase Part:BBa_J72173

AmilCP Part:BBa_K1467202

P35s Part:BBa_K1537015

T35s: GoldenBraid 2.0 Collection

References