Difference between revisions of "Part:BBa J04430:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
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===Applications of BBa_J04430===
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== Applications of BBa_J04430 ==
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<p><html><body><a href="http://2014.igem.org/Team:Warwick">Warwick iGEM 2014</a></body></html> used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.</p>
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<p>This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.</p>
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== Results ==
  
 
===User Reviews===
 
===User Reviews===

Revision as of 18:57, 15 October 2014


Applications of BBa_J04430

Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.

This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.

Results

User Reviews

UNIQc1bd9386bbdeead5-partinfo-00000001-QINU

No review score entered. arandall

1 mM IPTG induced expression using BL21 E. coli cells produces vibrant green cells from both 08 and 09 distributions.

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Smelissali

A lovely vibrant green under UV --Smelissali 19:45, 3 July 2006 (EDT)

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQc1bd9386bbdeead5-partinfo-00000005-QINU