Difference between revisions of "Part:BBa K1554000"
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<partinfo>BBa_K1554000 short</partinfo> | <partinfo>BBa_K1554000 short</partinfo> | ||
| − | The TA29 promoter is an anther-specific promoter. One of the most used strategies of biosafety in plants in male sterility. A cytotoxic gene (like barnase, ipt, RIP...) under the control of this promoter leads to a plant without fertile pollen. | + | The TA29 promoter is an anther-specific promoter. One of the most used strategies of biosafety in plants in male sterility. A cytotoxic gene (like barnase, ipt, RIP...) under the control of this promoter leads to a plant without fertile pollen. Our team used it in the construction of [https://parts.igem.org/Part:BBa_K1554004 Part:BBa_K1554004] and [https://parts.igem.org/Part:BBa_K1554005 Part:BBa_K1554005] |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The TA29 promoter permits the specific expression of genes under its regulation in the tapetum, a layer found in the anthers of flowring plants. One of the most used strategies related to biosafety in plants consists in producing male sterility. A cytotoxic gene (like barnase, ipt, RIP...) its control leads to a plant without fertile pollen, being unable to be self-pollinated and hence, unable to spread in an uncontrolled way in the field. Our team used it in the construction of [https://parts.igem.org/Part:BBa_K1554004 Part:BBa_K1554004] and [https://parts.igem.org/Part:BBa_K1554005 Part:BBa_K1554005], two biosafety modules for plants submitted to the Registry of Parts. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1554000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1554000 SequenceAndFeatures</partinfo> | ||
Revision as of 18:47, 15 October 2014
TA29 promoter
The TA29 promoter is an anther-specific promoter. One of the most used strategies of biosafety in plants in male sterility. A cytotoxic gene (like barnase, ipt, RIP...) under the control of this promoter leads to a plant without fertile pollen. Our team used it in the construction of Part:BBa_K1554004 and Part:BBa_K1554005
Usage and Biology
The TA29 promoter permits the specific expression of genes under its regulation in the tapetum, a layer found in the anthers of flowring plants. One of the most used strategies related to biosafety in plants consists in producing male sterility. A cytotoxic gene (like barnase, ipt, RIP...) its control leads to a plant without fertile pollen, being unable to be self-pollinated and hence, unable to spread in an uncontrolled way in the field. Our team used it in the construction of Part:BBa_K1554004 and Part:BBa_K1554005, two biosafety modules for plants submitted to the Registry of Parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]